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Blood adversely affects dentin bond strengths

 
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john kanca



Joined: 14 May 2005
Posts: 6346

PostPosted: Sun Jul 25, 2010 11:49 am    Post subject: Blood adversely affects dentin bond strengths Reply with quote

Oper Dent. 2010 May-Jun;35(3):330-6.

Effects of blood contamination on microtensile bond strength to dentin of three self-etch adhesives.
Chang SW, Cho BH, Lim RY, Kyung SH, Park DS, Oh TS, Yoo HM.

Abstract
This study evaluated the effects of blood contamination and decontamination methods during different steps of bonding procedures on the microtensile bond strength of two-step self-etch adhesives to dentin. Sixty extracted human molars were ground flat to expose occlusal dentin. The 60 molars were randomly assigned to three groups, each treated with a different two-step self-etch adhesive: Clearfil SE Bond, AdheSE and Tyrian SPE. In turn, these groups were subdivided into five subgroups (n = 20), each treated using different experimental conditions as follows: control group-no contamination; contamination group 1-CG1: primer application/ contamination/primer re-application; contamination group 2-CG2: primer application/contamination/wash/dry/primer re-application; contamination group 3-CG3: primer application/adhesive application/light curing/contamination/ adhesive re-application/light curing; contamina- tion group 4-CG4: primer application/adhesive application/light curing/contamination/wash/ dry/adhesive re-application/light curing. Composite buildup was performed using Z250. After 24 hours of storage in distilled water at 37 degrees C, the bonded specimens were trimmed to an hourglass shape and serially sectioned into slabs with 0.6 mm2 cross-sectional areas. Microtensile bond strengths (MTBS) were assessed for each specimen using a universal testing machine. The data were analyzed by two-way ANOVA followed by a post hoc LSD test. SEM evaluations of the fracture modes were also performed. The contaminated specimens showed lower bond strengths than specimens in the control group (p < 0.05), with the exception of CG1 in the Clearfil SE group and CG2 and CG3 in the Tyrian SPE group. Among the three self-etch adhesives, the Tyrian SPE group exhibited a significantly lower average MTBS compared to the Clearfil SE Bond and AdheSE (p < 0.05) groups. Based on the results of the current study, it was found that blood contamination reduced the MTBS of all three self-etch adhesives to dentin, and water-rinsing was unable to overcome the effects of blood contamination.
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Pcus



Joined: 17 May 2005
Posts: 434
Location: toronto

PostPosted: Mon Jul 26, 2010 1:18 pm    Post subject: Reply with quote

so what is your protocol when and if that happens
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john kanca



Joined: 14 May 2005
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PostPosted: Tue Jul 27, 2010 6:10 am    Post subject: Reply with quote

I would use the gel I am trying to get Ulttradent to make. It would be very effective.

So until then, H202 and/or alcohol is the best approach.
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john kanca



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PostPosted: Tue Jul 27, 2010 7:15 am    Post subject: Reply with quote

You know, Paul, either Surpass 1 or Simplicity 1 will also clean this up. Just use it like a cleaner and rinse it off.
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Pcus



Joined: 17 May 2005
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Location: toronto

PostPosted: Tue Jul 27, 2010 11:11 am    Post subject: Reply with quote

thanks John..that is good to know...when are you coming to TO, Rodney has been asking about you.
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john kanca



Joined: 14 May 2005
Posts: 6346

PostPosted: Tue Jul 27, 2010 12:10 pm    Post subject: Reply with quote

Pcus wrote:
thanks John..that is good to know...when are you coming to TO, Rodney has been asking about you.


Hoping Gary will set something up. Love to see Rodney again.
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