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Remineralization of vital dentin

 
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john kanca



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PostPosted: Sun Oct 04, 2009 10:41 am    Post subject: Remineralization of vital dentin Reply with quote

J Prosthet Dent. 1992 May;67(5):617-20.Links
Remineralization of etched dentin.Tatsumi T, Inokoshi S, Yamada T, Hosoda H.
Department of Operative Dentistry, Tokyo Medical and Dental University, Faculty of Dentistry, Japan.

To simulate a clinical cavity wall with the inner layer of carious dentin preserved, an artificially demineralized layer was made on the axial wall class V cavities of monkeys by a quick decalcifying solution. The cavities were then restored with a chemically adhesive composite resin after etching with a 40% phosphoric acid jelly etchant. Comparison of the Knoop hardness, the calcium concentration, and the scanning electron microscope observation of the dentin at the axial wall revealed that the dentin, demineralized by etching, was completely remineralized after 4 months.
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john kanca



Joined: 14 May 2005
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PostPosted: Sun Oct 04, 2009 10:41 am    Post subject: Reply with quote

Quintessence Int. 2001 Jul-Aug;32(7):561-70.Links
Remineralization across the resin-dentin interface: in vivo evaluation with nanoindentation measurements, EDS, and SEM.Akimoto N, Yokoyama G, Ohmori K, Suzuki S, Kohno A, Cox CF.
Department of Operative Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan. akimoto-n@tsurumi-u.ac.jp

OBJECTIVE: The purpose of this study was to evaluate the in vivo remineralization of the possible non-resin infiltrated hybridoid layer between the hybrid layer and the subjacent dentin substrate using nanoindentation, energy dispersive x-ray spectroscopy microanalyses (EDS), and scanning electron microscopy (SEM) technologies. METHOD AND MATERIALS: Twenty Class V cavities were placed in healthy adult monkey teeth. Each cavity was total etched with 37% phosphoric acid for 60 seconds, rinsed, and air dispersed, and SA-Primer was applied to the collagen layer. Cavities were divided into two groups: In group 1, Protect Liner (low-viscosity resin) and Clearfil AP-X (resin composite) were placed per manufacturer's directions, and no bonding agent was placed on the acid-etched interface. In group 2, Clearfil Photobond (bonding agent) was applied, and Protect Liner and Clearfil AP-X were placed as in group 1. Teeth were observed at 7 days (control) and 6 months by nanoindentation, EDS, and SEM. RESULTS: Six-month data showed an increased nanohardness in areas 5 pm adjacent to the demineralized or partially demineralized dentin interface. Following treatment with a conventional adhesive system on the acid-etched interface (group 2), there were increased nanohardness and calcium EDS measurements in the substrate just below the resin-dentin impregnated layer. CONCLUSION: Our 6-month in vivo nanoindentation and EDS data demonstrate that the non-resin infiltrated zone becomes remineralized following adhesive resin treatment.
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john kanca



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PostPosted: Sun Oct 04, 2009 10:53 am    Post subject: Reply with quote

Oper Dent. 2003 May-Jun;28(3):274-80.Links
Physiological remineralization of artificially demineralized dentin beneath glass ionomer cements with and without bacterial contamination in vivo.Kitasako Y, Nakajima M, Foxton RM, Aoki K, Pereira PN, Tagami J.
Tokyo Medical and Dental University, Graduate School, Department of Restorative Sciences, Cariology and Operative Dentistry, Tokyo, Japan. oztasnurhan@hotmail.com

This study evaluated the physiological remineralization of artificially demineralized dentin beneath glass ionomer cements with and without bacterial contamination. The artificially demineralized dentin was produced on 84 monkey teeth using a decalcifying solution. Half the samples were left open to the oral cavity for one week, then, all cavities were restored with two glass-ionomer cements: Fuji IX or Fuji II LC improved (n = 7). The nanohardness of the artificially demineralized dentin at 3, 90 and 360 days was measured using a nanoindentation tester (ENT-1100, Elionix) and compared statistically by two-way ANOVA and Fisher's PLSD test (p < 0.05). Each mineral (Ca, Mg, P, F) within the demineralized dentin was also analyzed using Electron Probe Microanalysis. For the samples, the mean nanohardness of the three-day samples was significantly lower than the 360-day samples (p < 0.05). Although there was no significant difference in the mean nanohardness within all the bacterially-contaminated groups through the experimental periods (p > 0.05), the mean nanohardness of the bacterial-contaminated samples were significantly lower than the non-bacteria-contaminated samples (p < 0.05). From the EPMA results, fluoride release from both cements to the bottom of the artificially demineralized dentin was detected within three days. Although Ca density was sparse within this demineralized dentin lesion, for the Fuji IX sample, a high Mg density within this lesion was detected at 360 days.



>> What this means is that remineralization is not material-specific
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